Identification of BMP-regulated genes in endothelial cells. (A) Hierarchical cluster diagram representing the expression pattern of 25 filtered genes with known angiogenic functions in endothelial cell isolations from wild-type and Bmpr2 KD mice. Each row represents one gene and each column one independent cell preparation. Fold changes of expression evaluated in both GeneChip and quantitative RT-PCR analyses are indicated. Negative value denotes downregulation in the KD sample; n.d., not determined. (B) Real-time RT-PCR analyses of selected genes in cultured mouse lung endothelial cells treated with 100 ng/mL BMP4 for the indicated time. Values are means plus or minus SD. (C) Immunoblot detection of total or phosphor-Ser785 integrin β1 and SEMA3C in the lung and cecum from WT or KD littermates. Detection of β-actin in the same blot indicates equal loading.