The FMNL1-PP2–specific T-cell clone shows peptide specificity and TCR dependency. (A) T2 cells were pulsed with a set of different peptides at 10 μM and investigated as targets for the FMNL1-PP2–specific T-cell clone in a ⁵¹Cr-release assay at an effector-target ratio of 7.5:1. Error bars indicate the standard deviation of tested duplicates. Results shown are representative of 2 experiments. (B) K562 and Daudi cells were used as target cells for the FMNL1-PP2–specific T-cell clone in a ⁵¹Cr-release assay at an effector-target ratio of 7.5:1. Error bars indicate the standard deviation of tested duplicates. Results shown are representative of 2 experiments. (C) Recognition of T2 cells pulsed with FMNL1-PP2 was inhibited by the monoclonal anti–HLA-A2 antibody (HB54) investigated by ELISA (IFN-γ release). Error bars indicate the standard deviation of tested duplicates. Results shown are representative of 2 experiments. (D) The cytotoxicity of the isolated T-cell clone against RCC26 could be inhibited by addition of cold T2 cells pulsed with FMNL1-PP2 but not pulsed with Flu. Error bars indicate the standard deviation of tested duplicates.