Expression of MHC-II molecules in TAMs from pancreatic cancers. DcR3 expression in human pancreatic cancers was visualized by immunohistochemistry (IHC) staining using anti-DcR3 mAb and the ultra-View Universal DAB detection kit (A,B). Double staining of CD68 (brown) and HLA-DR (pink) was performed with the Ventana iVIEW DAB and the Enhanced V-Red detection kit, respectively (C-D). Circled cells correspond to small lymphocytes as an HLA-DR–positive control, arrowheads indicate HLA-DR–negative TAMs (strong brown color) in DcR3^high sample (C), and arrows point to HLA-DR–positive TAMs (weak brown color interdispersed with pink color) in DcR3^low sample (D) (magnification 10 × 60). (E) Effects of DcR3 expression on the relapse-free survival of pancreatic cancer patients. Kaplan-Meier survival curves are shown for patients from DcR3^high (3+ and 2+) and DcR3^low (1+ and 0) groups in green and blue, respectively.