TNF induction of jagged-1 expression depends on NFκB activity. (A) EC-coated beads were established in fibrin gels and treated with TNF (10 ng/mL) or TNF + NFκB inhibitor (100 nM). Sprouts were counted and data are presented as means plus SD; *P < .005 relative to control, by Student t test. One of 3 similar experiments. (B) ECs were transfected with GFP- or DN-IKKβ expression plasmids and cultured with or without TNF (10 ng/mL). RNA was harvested at 12 hours and gene expression examined by qRT-PCR. Means and SD are shown. *P < .001 relative to control, by Student t test. One of 2 similar experiments. (C) ECs were transfected with GFP- or DN-IKKβ expression plasmids and cultured with TNF (10 ng/mL) for the indicated times. RNA was harvested and gene expression examined by qRT-PCR. One of 2 similar experiments. (D) ECs were transfected with GFP- or CA-IKKβ expression plasmids and cultured for the indicated times. RNA was harvested and gene expression examined by qRT-PCR. One of 2 similar experiments. (E) ECs were cultured in the presence or absence of the p38 inhibitor SB203580 and/or TNF (10 ng/mL) for 18 hours and jagged-1 expression was assayed by qRT-PCR. Means and SD are shown. *P < .01 by Student t test. One of 3 similar experiments. (F) ECs were cultured in the presence or absence of the p38 inhibitor SB203580 and jagged-1 expression was assayed by qRT-PCR at various times after the addition of TNF (10 ng/mL). Means and SD are shown. *P < .02 by Student t test. One of 3 similar experiments.