The chromatin association of FAAP24 depends on FANCM. (A) HeLa cells transfected with control or FANCM siRNA for 72 hours were treated with MMC (50 ng/mL for 17 hours) or HU (2 mM for 17 hours) and harvested. Fractionated extracts were immunoblotted for the indicated proteins. Mus81 was used a loading control for both fractions. (B) HeLa cells transfected with the indicated siRNAs for 72 hours were treated with MMC (50 ng/mL for 17 hours) and harvested. Fractionated extracts were immunoblotted for the indicated proteins. RPA70 was used a loading control for both fractions.