FANCM requires FAAP24 for its stable association in chromatin. (A) HeLa cells were transfected with indicated siRNAs for 72 hours, and then whole-cell extracts were immunoblotted. (B) HeLa cells transfected with indicated siRNAs for 72 hours were treated with MMC (50 ng/mL for 17 hours) or HU (2 mM for 17 hours), and whole-cell extracts were immunoblotted for the indicated proteins. (C) HeLa cells transfected with indicated siRNAs for 72 hours, and then incubated with MMC (50 ng/mL) for 7 hours, 12 hours, and 20 hours. Cells were then extracted and fractionated, and each fraction was immunoblotted for the indicated proteins.