FK778 inhibits the cytokine-producing potential of allogeneic stimulated CD4⁺ T cells. PBMCs were stimulated with allogeneic stimulator cells for 7 days in the presence or absence of FK778. After the 2- to 3-day rest period, the cytokine-producing potential was investigated by intracellular cytokine staining using flow cytometry. Analysis was performed on gated CD3⁺CD8⁻ cells. The fluorescence intensity of the particular cytokine staining (x-axis) is plotted against the forward scatter of the cells (y-axis). The percentage of cytokine-positive cells is presented in each plot. One representative experiment of 3 is presented.