Kinetics of activation-induced antigens expressed on allogeneic monoclonal and oligoclonal T-cell cultures. MLLC or MLTC responders were generated by 4 weekly in vitro stimulations of healthy donor CD4 or CD8 T cells with allogeneic AML or RCC cells, respectively. HLA class I and II genotypes of donor and stimulator cells were either fully matched or differed at a single allele or haplotype. CTL clone E77 recognizes mismatch HLA-Cw*0702 on allogeneic RCC cells. Flow cytometry analyses were performed on clonal and oligoclonal T-cell cultures before (day 0) and on days 1 to 6 after rechallenge with AML or RCC cells. Antibodies used were conjugated to FITC (CD4 and CD8) or PE (activation markers). Data represent percentages of T cells with FITC or PE costaining. Similar results were obtained in at least 3 independent experiments per each test condition.