Region of duplication in FLT3/ITD. (A) Proportion of patients with involvement of specific aa residues in the duplicated region. Codons 591 to 597 are shown in darker bars, and specific aa residues in this region are shown in the center inset. (B) The region of duplication in all 77 patients are sorted by ITD length (longest to shortest) from top to bottom. The area of duplication of the major ITD peak is shown in dark highlighted area. In patients with additional ITDs, the duplicated region is shown in red with the area of overlap in blue. Codons 591 to 597 are shown in shaded box. (C) FLT3 structure showing the location of the JM domain and Y597. Ribbon representation of the crystal structure of FLT3 previously solved by Griffith et al.14 The kinase domain N-lobe is colored green and the C-lobe, blue. The activation loop is colored orange and the JM domain, yellow. Tyrosines 597, 591, and 589 are shown in stick format. The exploded view shows the molecular surface of the kinase domain and JM domain side-chain residues Y591 to Y597. Tyrosine 597 is depicted with space-filling spheres. This figure was made using the program Pymol (www.pymol.org).
