Flow cytometric analysis of human T cells in the peripheral blood and spleen in NOG mice given intravenous transplants of human CB-derived CD34⁺ cells. (A-C) Representative profiles of the mice 2 months (A), 4 months (B), and 7 months (C) after transplantation. The ratio of human to murine CD45⁺ cells and that of human CD3⁺ to CD19⁺ cells show an incremental increase in human CD45⁺ cells and human CD3⁺ cells from 2 to 7 months. (D) Change of net percentages of human CD3⁺ T cells among human CD45⁺ cells in peripheral blood and the spleen from 38 mice 39 to 213 days after transplantation. (E) CD45RA is more efficiently expressed than CD45RO on human CD3⁺ T cells in spleen. A gate was set on the human CD45⁺ population. The fluorescence-activated cell sorting (FACS) profile is representative of 1 in a group of 5 mice.