Flow cytometric analysis and immunohistochemical analysis of the expression of myelomonocytic markers in hNOG mice 4 months after transplantation. (A) Human CD14⁺ monocytes/macrophages are recognized in peripheral blood, the spleen, and BM. (B-G) Immunohistochemical findings from serially sectioned spleen for the expressions of human CD45 (B), human CD20 (C), human CD3 (D), murine FDC (E), human CD205 (F), and human CD68 (G). (H) Human CD68⁺ macrophages are also detected in the medulla of the LN and lung. Visualization was performed with BCIP (B-D, F-G), DAB (E), and AEC (H). Original magnification, ×100.