Chimerism response following CD8-depleted DLIs. Donor chimerism results (%) were obtained from unseparated peripheral blood leukocytes (-□-), purified CD4 T cells (-⋄-), and purified CD8 T cells (-♦-) of patients 3, 8, and 10. Stars indicate the time of first GVHD diagnosis. Longitudinal bars indicate duration of systemic immunosuppressive treatment (CsA, cyclosporine A). The dotted lines are CD3 T-cell counts in peripheral blood. Mixed T-cell chimerism was not detectable in unseparated peripheral blood leukocytes if the proportions of CD4 and CD8 T cells were below the detection limit of the chimerism assay (5%).