Gal-2 and Gal-4 induced PS exposure in MOLT-4 and HL60 cells. (A) MOLT-4 cells were incubated with ALEXA 488–Gal-2, and (B) HL60 cells were incubated with ALEXA 488–Gal-4, followed by flow cytometric analysis. NANase indicates prior treatment of cells with A urefaciens neuraminidase (100 mU) for 1 hour at 37°C. (C-D) HL60 cells or MOLT-4 cells were incubated with either 10 μM Gal-2 (C) or 3 μM Gal-4 (D) for 4 hours followed by flow cytometric analysis for PS externalization. NANase indicates prior treatment of cells with A urefaciens neuraminidase (100 mU) for 1 hour at 37°C. Error bars represent the standard deviation of duplicate samples.