Gal-1 and Gal-2 but not Gal-4 induce Ca²⁺ flux in both resting and activated neutrophils. Resting neutrophils were treated with 10 μM Gal-1 (A), 10 μM Gal-2 (D), or 5 μM Gal-4 followed by 10 μM Gal-1 (G), followed by detection for changes in intracellular Ca²⁺ levels. Resting neutrophils were activated with fMLP followed by treatment with either 10 μM Gal-1 (B), 10 μM Gal-2 (E), or 5 μM Gal-4 followed by 10 μM Gal-1 (H), followed by detection for changes in intracellular Ca²⁺ levels. fMLP-activated neutrophils in parallel assays were treated with 10 μM Gal-1 (C), 10 μM Gal-2 (F), or 5 μM Gal-4 (I) for 4 hours followed by detection of PS exposure. Error bars represent the standard deviation of duplicate samples.