Analyses of hematopoietic cells of JAK2^V617F transgenic mice. (A) Blood cell smears stained with the Wright and Giemsa reagent. Arrows point to platelets. The big arrow indicates a giant platelet. (B) Blood cell counts of a representative nontransgenic control (—) and 3 line A transgenic ( and …) mice at different ages. (C,D) Flow cytometric analyses of peripheral blood, bone marrow, and spleen cells from nontransgenic and line A transgenic mice. Cells were stained with phycoerythrin-conjugated anti-B220 and fluorescein isothiocyanate-conjugated anti-Gr-1, anti-Ter119, anti-CD71, or anti-CD41. Percentages of cells in each quadrant are indicated.