Dynamics of antileukemia immune responses. (A) Immune responses in 4 patients via IFN-γ ELISPOT analysis. Stimulated remission samples (■), leukemic cells alone (□), and remission samples alone (). Background levels are connected by lines (*P < .05, statistically significant responses over background). Ratio of the tumor load measured by molecular responses (bcr-abl transcript/bcr-abl control gene, left panel, ○) and levels of Ph⁺ cells in chromosome analysis (right panel, ▵) at the corresponding time points. (B) TNF-α (■) and IFN-γ(▾) responses by CD4⁺ and CD8⁺ T cells (CFC) from patients P4 and P13 over time. Leukemic cells alone (TNF-α (■), IFN-γ(▾)) and remission samples alone (TNF-α (), IFN-γ(). Background levels are connected by lines (*P < .05, statistically significant responses over background). (C) Tumor load over time from patients P5(▴), P6(▾), P7 (♦), P8 (●), connected by solid lines, and patients P9 (□), P11 (▿), and P14 (○), connected by dotted lines. Error bars indicate ranges of triplicate measurements. Results are representative of 2 or 3 independent experiments.