Responses of T-cell clones to tumor lysate, but not to TAAs. (A) TNF-α and IFN-γ responses of 2 CD4⁺ and 2 CD8⁺ T-cell clones stimulated with autologous leukemic cells (■) via multiplex cytokine array compared with unstimulated T-cell clones () and T-cell clones stimulated with remission sample (). (B,C) Responses in T-cell clones stimulated with remission sample pulsed with lysate from autologous leukemic cells (■) and remission sample pulsed with lysate from the AML cell line THP1 () using CFC for TNF-α and IFN-γ (B) and blocked with anti-CD4 and anti-MHC II antibodies or anti-CD8 and anti-MHC II antibodies, respectively (C). Results were compared with PHA and ionomycin-stimulated T-cell clones. (D) T-cell responses in CD8⁺ T-cell clones pulsed with HLA-A0201 restricted peptides from LAAs and TAAs using ELISPOT assay compared with PHA stimulated CD8⁺ T-cell clones, and CD8⁺ T-cell clones stimulated with a peptide from the melanoma-associated antigen gp100. Results are shown as median and range.