FOXP3⁺ Tregs are resident in healthy human skin. Sections of healthy human skin were costained with directly conjugated antibodies to CD3 (red) and FOXP3 (green). FOXP3⁺ Tregs were clearly visible as cells with red membranes and green nuclei. (A) Two T cells present within the epidermis are shown, 1 regulatory (white arrow) and 1 nonregulatory. In general, Tregs were found in the same locations as non-Tregs, located near blood vessels and appendages within the dermis. (B) A particularly brisk infiltrate of T cells containing Tregs surrounding a blood vessel. Such infiltrates can be characteristic of normal human skin.