Figure 2
Figure 2. Inhibition of Stat5 phosphorylation in the presence of MSCs. . / Western blot analysis of Stat5 phosphorylation. Splenocytes (2 × 106) were activated with anti-CD3/CD28 beads in the presence or absence of 1 × 105 MSCs. After 48 hours, splenocytes were collected, lysed, and analyzed by Western blotting. Each lane contains 20 μg protein. Western blotting with anti-Stat5 is shown as a loading control. Shown are representative results from more than 5 experiments.

Inhibition of Stat5 phosphorylation in the presence of MSCs.

Western blot analysis of Stat5 phosphorylation. Splenocytes (2 × 106) were activated with anti-CD3/CD28 beads in the presence or absence of 1 × 105 MSCs. After 48 hours, splenocytes were collected, lysed, and analyzed by Western blotting. Each lane contains 20 μg protein. Western blotting with anti-Stat5 is shown as a loading control. Shown are representative results from more than 5 experiments.

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