TGF-M-DCs respond to LPS with markedly enhanced phosphorylation of members of MAPK pathway (ERK, JNK, and p38) compared with TGF-GM-DCs. TGF-M-DCs and TGF-GM-DCs were either not treated (as a control) or treated side by side for 15 and 30 minutes with LPS. Cell lysate was subjected to Western blotting using rabbit polyclonal antibodies specific for p-ERK, -JNK, or -P38. PVDF membranes were stripped and reprobed with a rabbit polyclonal antibody specific for total ERK as a loading control.