SlanDCs are mobilized by G-CSF and display an unaltered phenotype. Peripheral blood samples were collected from the same donors before and after G-CSF treatment. (A) Absolute cell counts of slanDCs (M-DC8⁺), CD1c⁺ DCs (lin⁻, HLA-DR⁺, CD11c⁺), and pDCs (lin⁻, HLA-DR⁺, CD11c⁻) were determined as described in “Materials and methods” (n = 13). Individual values and the median are shown. P values were determined using paired t tests. (B) The phenotypic analysis of slanDCs before and after G-CSF administration is compared. Results of one representative donor are shown (n = 5). Staining of PBMCs and gating were performed as described.^12,13