The frequency of LMP2-specific T cells increased after infusion of polyclonal LMP2-specific CTLs. LMP2 multimer analysis was also used to compare the frequency of LMP2-specific CTLs before and after infusion. The average number of LMP2 and CMV multimer-reactive cells per 106 CD8+ T cells are shown for 1 patient before and after infusion (A). This patient was representative of the 10 patients who received CTLs where specific LMP2 epitopes had been characterized. In these 10 patients in whom the HLA-restricted LMP2 peptide(s) were available, peripheral blood T cells were incubated with LMP2pepmix, and the number of IFN-γ spot-forming cells per 2 × 105 mononuclear cells was measured (B). In the 6 patients in whom LMP2 peptides were not detected, peripheral blood T cells were incubated with autologous LCLs, and the number of IFN-γ spot-forming cells per 2 × 105 mononuclear cells was measured (C).
