After aBMT, the early relative increase in CD4⁺CD25⁺ T cells in spleen and lymph nodes is initially not accompanied by an increase in Foxp3-expressing cells. Repopulation of CD4⁺CD25⁺Foxp3⁺ cells in spleen and lymph nodes (LNs). (A) Before BMT and on days 35, 42, 55, and 81, N = 3 animals were killed per group, and FACS analysis was performed on the relative amount of CD25⁺ and CD25⁺ Foxp3⁺ cells within the CD4⁺ T-cell population, shown (+ SEM). (B) The relative amount of Foxp3⁺ cells within the CD4⁺CD25⁺ T-cell compartment was determined by FACS, shown (+ SEM).