Ova-pulsed neutrophils fail to induce cross-tolerance in vivo. (A) β2-Microglobulin^−/− mice were injected with 3 × 10⁶ naive OT1 CD8⁺ T cells. One day later, mice were injected with PBS, Ova, or 3 × 10⁶ Ova-pulsed LB27.4, BMNs, PENs, or DCs. At day 13, mice were injected with Ova-pulsed DCs (□) or unpulsed DCs (■), plus CFA. Three days later, spleen CD8⁺ T cells were purified and IL-2 and IFN-γ production was analyzed by ELISA 24 hours after in vitro restimulation. (B) IFN-γ and IL-2 production was determined by ELISA at day 16. Results are expressed in ng/mL (IFN-γ) and in pg/mL (IL-2) as mean (± SD) of 3 separate experiments. Mann-Whitney test was performed. The data were statistically significant for BMNs, PENs, and DCs (*P < .03) when compared with controls.