Characterization of PDMV-induced augmentation of IgG production. (A) Time course of PDMV response. CD154^−/− mice injected with PDMV pellet from 5 × 10⁸ B6 or CD154^−/− platelets. Serum collected on days 3, 5, 7, and 9. Total IgG quantified by ELISA using commercial mouse antiadenovirus IgG standard (Wilcoxon rank sum test: *2-tailed P = .020, **2-tailed P = .012 comparing the B6 PDMV time point to the corresponding CD154^−/− PDMV time point). (B) Dose-response study. Neat samples contain PDMV from 4.5 × 10⁸ activated platelets. Dilutions made from resuspended PDMV pellets. Total IgG quantified by ELISA using commercial mouse antiadenovirus IgG standard (Wilcoxon rank sum test: *2-tailed P = .012, **2-tailed P = .012; ns indicates not significant). (C) Analysis of antibody isotypes produced. Samples pooled from neat groups in dose-response experiment. ELISAs were performed for each antibody isotype. Each experiment was performed with 5 mice per group and repeated once.