American Society of Hematology

Figure 6

$Figure 6. CCL5-inducible protein translation enhances mRNA association with polyribosomes. PB T cells were pretreated with either DMSO (carrier) or 50 nM rapamycin for 1 hour, followed by 10 nM CCL5 for 1 hour. Cells were harvested and lysed, and lysates were layered onto a sucrose gradient. Fractions were collected after centrifugation, and RNAs were extracted and quantified at optical density (OD) 254 nm. A representative gel profile of fractions from untreated cells is shown to visualize the distribution of 5S, 18S, and 28S rRNAs as an indicator of the polyribosome integrity (top panel). OD readings for each fraction were plotted as a percentage of the total RNA of all fractions and are shown as a function of gradient depth (bottom panel). Actively translated mRNA is associated with high-molecular-weight polysomes deep in the gradient (shaded region). Data are representative of 2 independent experiments.$

CCL5-inducible protein translation enhances mRNA association with polyribosomes. PB T cells were pretreated with either DMSO (carrier) or 50 nM rapamycin for 1 hour, followed by 10 nM CCL5 for 1 hour. Cells were harvested and lysed, and lysates were layered onto a sucrose gradient. Fractions were collected after centrifugation, and RNAs were extracted and quantified at optical density (OD) 254 nm. A representative gel profile of fractions from untreated cells is shown to visualize the distribution of 5S, 18S, and 28S rRNAs as an indicator of the polyribosome integrity (top panel). OD readings for each fraction were plotted as a percentage of the total RNA of all fractions and are shown as a function of gradient depth (bottom panel). Actively translated mRNA is associated with high-molecular-weight polysomes deep in the gradient (shaded region). Data are representative of 2 independent experiments.

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