Netrin-4 activates intracellular signaling pathways of HMVEC-dLys. Determination of the phosphorylation of Akt (Ser 473; Serine 473, panels A and D, respectively), p42/p44 (Thr 202/Tyr 204; Threonin 202/Tyrosine 204, panels B and E, respectively), and Ribosomal protein S6 (Ser 235/236; Serine 235/236, panels C and F) by Western blotting after Netrin-4 treatment of HMVEC-dLy. Experiments were performed in triplicate.