HPCs derived from HOXB4-transduced ES cells contribute to multilineage hematopoiesis in Rag2^−/−γ_c^−/− mice. (A) Flow cytometric analysis of peripheral blood, spleen, and bone marrow of a representative Rag2^−/−γ_c^−/− mouse 56 days after HPC transplantation (n = 5). Numbers represent percentages of cells to the total GFP-expressing cell population. The majority of the HPC-derived cells were Gr-1 positive and therefore myeloid. (B) Flow cytometric analysis of hematopoietic cells in peripheral blood, spleen, and bone marrow cells of chimeric Rag2^−/−γ_c^−/− mice transplanted with either HPCs (56 days after transplantation [Tx]) or with marrow cells of GFP transgenic mice (60 days after transplantation). Donor-derived cells were predominantly Gr-1 positive after HPC transplantation compared with B220-positive–expressing cells after BMT (BM Tx). Error bars represent SD.