Mammalian reovirus infects CBMCs and induces CXCL8 production. CBMCs were infected with mammalian reovirus type 3 Dearing at an MOI of 2, 20, or 200 pfu/cell, or with UV-inactivated reovirus at an MOI of 20 pfu/cell for 24 hours. (A) CBMCs were stained with an antireovirus Ab by immunofluorescence and detected by flow cytometry after exposure to reovirus (thick black line) or medium control (filled gray). Histograms are representative of 2 to 3 experiments using different CBMC cultures. (B) Supernatants from these infections were also analyzed for CXCL8 content by ELISA. Bar graph shows mean (± SEM) of 3 to 9 experiments. *P < .05, **P < .05, compared with CBMC medium. (C) Human primary HDFs, and CCL-204 lung fibroblasts were grown to confluence in 6-well plates. These, along with CBMCs, were treated with medium control or with reovirus at an MOI of 2 or 20 pfu/cell for 24 hours under similar FBS conditions. CXCL8 content was measured in the supernatants by ELISA. Graphs show the mean (± SEM; ng/106 cells) of the increase in CXCL8 content over medium control of 3 to 6 experiments. CCL-204 did not produce CXCL8 above medium control after treatment with 2 MOI of reovirus.
