Overexpression of scl-β expands erythroblasts but not trunk angioblasts and rescues runx1 expression in etsrp morphants when angioblasts are restored by fli1a overexpression. (A-F) flk1 and gata1 expression in the PLPM at 14 hpf, flatmount, anterior to left. (A-C) flk1 expression in trunk angioblasts (arrowheads) is broader in embryos injected with the mRNA of scl-α (B) but not scl-β (C). (D-F) gata1 expression in erythroblasts (arrowheads) is broader in embryos injected with the mRNA of either scl-α (E) or scl-β (F). (G-I) Fluorescent images of flk1-GFP at 30 hpf in control (G), etsrp MO (H), and etsrp MO + fli1a mRNA (I). Arrowheads denote axial vessels, and arrows denote ISVs (G,I). (J-M) runx1 expression in the ventral wall of the DA (arrowheads) at 30 hpf in control (J), etsrp MO (K), etsrp MO + fli1a mRNA (L), and etsrp MO + scl-β and fli1a mRNA (M).