Figure 1
Analysis of PNGase F digested VWF. (A) Equal amounts of digested and nondigested VWF diluted in PBS were coated overnight at 4°C onto microtiter plates and N-linked glycans detected with concanavalin A diluted 1/1000 in Tris-buffered saline, pH 7.4, supplemented with 1 mM CaCl2. Error bars represent mean and SD. (B) Reducing 7% SDS-PAGE analysis of denatured and nondenatured VWF digested with PNGase F. Nondigested VWF (lane 1), nondenatured VWF (lane 2), and denatured VWF (lane 3). (C) Binding of pdVWF and PNG-VWF to immobilized type III collagen. (D) Multimer analysis of PNGase F-digested VWF in 1.6% agarose multimer gels. pdVWF (lane 1); PNG-VWF (lane 2).
