HOXA activation in SET-NUP214⁺ T-ALL. (A) Relative HOXA9 expression levels by RQ-PCR for MLL-rearranged, CALM-AF1⁰⁺, inv(7)(p15q34), TAL1-, LMO2-, HOX11L2-, or HOX11-rearranged patients and T-ALL cell lines including LOUCY, MOLT13, SKW3, HPB-ALL, HSB2, and PEER. (B) Comparison of HOXA9 expression levels between the HOXA T-ALL subgroup (MLL, CALM-AF10, inv(7)(p15q34), and SET-NUP214; n = 10) and other T-ALL subgroups (TAL1, LMO2, HOX11L2, or HOX11). The horizontal lines represent the medians. (C) Relative expression levels of HOXA genes by RQ-PCR for the 3 SET-NUP214⁺ patients with T-ALL, the LOUCY cell line, the patient with AUL, and SKW3. (D) Heatmap of the 20 significant and differentially expressed probesets with a false discovery rate (FDR) lower than 5% for the HOXA cluster compared with the other patients with T-ALL.