Effects of dasatinib on histamine release in blood basophils of allergic donors. (A) Basophils of allergic donors (n = 11) were incubated with control medium or dasatinib (1 μM) at 37°C in 5% CO2 for 30 minutes. Then, cells were washed and exposed to allergens (1 μg/mL) for 30 minutes. Thereafter, basophils were centrifuged and the cell-free supernatants and lysates examined for the content of histamine by RIA. The inhibitory effect of dasatinib on histamine release was found to be significant (P < .05). (B-D) Dose-dependent effects of dasatinib on histamine release provoked by the allergens rBet v 1 (birch pollen), nDer p 1 (house dust mite), and rPhl p5 (timothy grass). Basophils from allergic donors were preincubated in control medium or various concentrations of dasatinib (0.01-1 μM) for 30 minutes (37°C). Then, cells were washed and exposed to rBet v 1, nDer p 1, or rPhl p 5 (each 1 μg/mL) for 30 minutes as indicated. After centrifugation at 4°C, cell-free supernatants and cell lysates were analyzed for histamine content by RIA. Results show the percentages of released histamine and represent mean (± SD) values from triplicates.
