MMSET interacts cofactors and histone deacetylases. (A) Total lysates of HEK293T cells transfected with HDAC1 or HDAC2 were immunoprecipitated with anti-MMSET antibody and probed with antibodies to epitope-tagged deacetylases. (B) Nuclear extracts from H929 and KMS11 cells were immunoprecipitated with anti-HDAC 1 and 2 antibodies and probed with anti-MMSET. (C) Total cell lysates from untransfected HEK293T cells and cells transfected with MMSET-II were immunoprecipitated with anti-mSin3A or anti-MMSET antibodies and analyzed by immunoblotting with the anti-MMSET and anti-Sin3A antibodies, respectively. (D) A Gal luciferase reporter gene was coexpressed in 293T cells along with Gal4-HDAC1 or Gal4-HDAC2 in the presence or absence of MMSET. Error bars represent SD. (E) MMSET-I and MMSET-II coimmunoprecipitate with the histone demethylase LSD1. Nuclear extracts (NE) from HEK293T cells were coimmunoprecipitated with either anti-LSD1 antibody or rabbit IgG, and immunoblotted with anti-MMSET antibody.