Depletion of MMSET affects myeloma cell viability. (A) KMS11 cells transduced by nucleoporation with a control shRNA or 2 shRNA constructs directed against MMSET were grown in culture for 5 days, sorted for GFP expression, lysed, and immunoblotted with anti-MMSET antibody. (B) t(4;14)-positive and t(4;14)-negative myeloma cell lines were nucloeporated with the indicated shRNA, grown in culture for 5 days, and stained for annexinV expression. Histograms of annexin V staining of GFP⁺, propidium iodine-negative cells are displayed along with the percentage of cells staining positive for annexin V.