Second skin graft survival and donor-specific antibody generation after the second skin grafting. (A) At 5 to 7 weeks after the first skin graft, a second skin graft was transplanted onto experimental B6 mice treated with anti-CD154, anti-αβ TCR, and/or both mAbs at the time of the first skin grafting. Mice without mAb treatment at the first skin graft served as controls. In mice treated with both mAbs, 6 of 8 mice had the first skin graft intact when the second skin graft was performed. Survival reflects days after placement of second skin grafts. (B) Sera were collected at weeks 2 and 4 after second skin grafting and tested with FCXM assay. The sera collected before second skin grafting served as controls. ♦ represents an individual sample, and the short bar represents the average for each group. Pre indicates pretreatment.