Memory T-cell development. B6 mice treated with anti-CD154 mAb (days 0, 3) were given transplants of BALB/c skin grafts (day 0). Naive, non-Ab–treated, and control hamster IgG–treated B6 mice served as controls. Recipient peripheral blood was collected at selected time points up to 30 days after skin grafting and stained with anti-CD44–FITC, anti-CD62L–PE, anti-CD4–PerCP, and anti-CD8–APC mAbs. n = 8 mice per group. (A) The kinetics of percentage of effector CD8⁺ T cells was analyzed (percentage CD44^high/CD62L^low/− cells in the CD8⁺ population). Data are presented as averages plus or minus SD. (B) A representative flow cytometry dot plot of each group for CD8⁺/CD44^high/CD62L^low/− effector cells at day 10 after skin grafting. (C) The kinetics of percentage of CD44^high/CD62L^high (central memory T-cell phenotype) in the CD4⁺ population. Data are presented as averages plus or minus SD. (D) A representative flow cytometry dot plot from each group for CD4⁺/CD44^high/CD62L^high cells at day 10 after skin grafting. Results are representative of 3 experiments.