Reintroduction of SCL using TAT-mediated protein transduction. (A) Depiction of full-length and truncated TAT-SCL fusion proteins. (B) (i) Coomassie staining and (ii) Western blot (anti-His) of bacterially expressed TAT-SCL. M indicates marker; −I, before induction; +, induction; IPTG, 1 hour; P, pellet; SN, supernatant; W1,2, wash with 10 mM imidazole; E1,2, 250 mM. (C) Western blot of YS1 cells with anti-SCL after transduction with recombinant TAT-SCL protein.