Telomere length measurements in DC CD34⁺ cells by STELA. (A) Representative STELA blot. Multiple PCR replicates for each DC sample of day-4 cultured CD34⁺ mPB cells were amplified at an estimated concentration of 10 molecules per well, loaded blind and resolved on 0.7% agarose, Southern blotted, and detected with an XpYp-specific probe. Amplicons were counted into size bins to estimate average telomere length. (B) Distribution of telomere lengths in CD34⁺ cells of one control (IWN1) and 3 DC patients. The average telomere length for each patient (± SEM) and the number of telomeres analyzed are given. Black portions of the graph represent ultrashort telomeres, defined by their length relative to the majority of telomere molecules.