Assessment of IL-13–induced eosinophil recruitment in Pirb^−/− mice. Mice received intranasal treatment of 10 μg/day every other day of either IL-13 or control saline solution. The total cell count (A) and differential cell counts (B) in BALF 48 hours after the final saline or IL-13 challenge are shown. Data are expressed as means plus or minus SD; *P < .05; **P < .01; ns indicates nonsignificant; n = 3 (4-6 mice/experimental group). Quantification of eosinophil numbers in the lung (C) and esophagus (D) was assessed by digital morphometric analysis. Data are expressed as means plus or minus SD; *P < .05; **P < .01 when comparing IL-13–treated WT and Pirb^−/− mice; n = 3 experiments (4-6 mice/experimental group). The levels of eotaxin-1 and eotaxin-2 (E,F) were assessed in the BALF of WT and Pirb^−/− mice using a commercial ELISA. Data represent means plus or minus SD of n = 3 experiments (4-6 mice/experimental group).