Erythemic appearances in Phd1/3 DKO and Phd2 CKO mice. (A-D) Erythemic external appearance (reddish paw and snout, A and B, respectively), and enlarged liver (C) and spleen (D) in Phd1/3 DKO mice. WT, wild-type; DKO, Phd1/3 double knockout mice. (E-H) Phd2 CKO mice and Phd2^f/f control mice. Abdomens (E) and paws (F) at approximately 6 weeks after tamoxifen treatment of both CKO and Phd2^f/f mice. Note the necrotic intestine and red paw in Phd2 CKO mice. Left, Phd2^f/f; right, Phd2 CKO. (C) Spleens. Top, Phd2^f/f; bottom, Phd2 CKO. (D) Livers. Left, Phd2^f/f, right, Phd2 CKO. Images were taken with a CoolSNAP charge-coupled device digital camera (Photometrics-Roger Scientific, Tucson, AZ) attached to a Leica MZFLIII stereomicroscope (Leica Microsystems, Heerbrugg, Switzerland). A single objective lens (Leica Plan 1.0, 10× magnification, Leica Microsystems) was used to obtain the images. Openlab (Improvision, Lexington, MA) was used for initial image acquisition, and Photoshop 7.0 (Adobe Systems, San Jose, CA) was used for subsequent processing.