Figure 5
Analysis for hematopoietic progenitors and hematopoietic stem cells in Phd2 CKO mice. (A-C) Flow cytometry analyses. (A) Erythroid (Ter119+); (B) myeloid (CD11b+); (C) HSC (Lin− Sca-1+ CD117+). Bone marrow (BM), spleen, and liver samples were analyzed. Total cell numbers in each organ are shown. n = 6 to 7. P values are indicated above the bars. (D) Methylcellulose colony formation assay. Genotypes are indicated at the bottom. f/f and CKO refer to Phd2f/f and Phd2 CKO mice, respectively. Number of colonies per 105 cells are presented, n = 6; P values are indicated in the figure. Error bars represent SEM.
