Normal hematologic parameters in mice heterozygous for Phd2 knockout. (A-E) Phd2 conditional knockout mice and controls. (A) Hematocrit values. (B) Hemoglobin concentrations. (C) Number of red blood cells. (D) White blood cells. (E) Platelets. Two weeks before tamoxifen administration, baseline measurements were performed (Tam, −). Measurements were taken again 2 weeks after tamoxifen administration (Tam, +). CreERT2 status is indicated as Cre − or +, representing absence or presence, respectively. Phd2f/− mice carrying CreERT2 and fed with tamoxifen are also designated as CKO, for conditional knockout. These are similar to Phd2 CKO mice used in other experiments except that one of the allele here was − before tamoxifen treatment; it was inherited as a germline mutation. For (A) to (E), n = 3 to 7; *P < .05. (F) Hematologic analysis of germline heterozygotes at more than 20 weeks of age. n = 5 to 6. Error bars represent SEM.
