Figure 7
![Figure 7. Molecular analysis for Phd2 CKO and Phd1/3 DKO mice. (A) Autoradiography of [35S]methionine-labeled PHD1, 2 and 3 produced by IVTT. Free, unincorporated [35S]methionine. (B) Western blotting analysis for PHD1, -2, and -3 in various tissues from wild-type adult mice. Equal amounts of IVTT-produced PHD1, -2, and -3 proteins were loaded as controls. Note PHD1 had 2 bands because of alternative translational initiation. (C) Anti-PHD2 Western blots for Phd1/3 DKO tissues. (D) Anti-HIF-1α and HIF-2α Western blots for Phd2 CKO and Phd1/3 DKO mice. (E,F) Q-PCR analysis for Epo mRNA. f/f, Phd2f/f; CKO, Phd2 CKO; WT, wild-type; DKO, Phd1/3 DKO. n = 6. (F) The scale in panel E was reduced. **P < .01; *P < .05. Error bars represent SEM.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/111/6/10.1182_blood-2007-09-114561/6/zh80040815160007.jpeg?Expires=1735572508&Signature=NkJ34blPvbDAXYvqIpKRsytb01AuYKc~X1TjFItQ6teJYkathrNlCtGSu281~4rilXRb436U-ceDPuE1exRCun62PY~KdBEGQy1GlakII1nn5sZC85CHAxL8kgqGTinlKLFs9QtqFCXu4QIhH4xSfD-SlJ0U8V1LZAmJ57~iU5DaG3Rlr8iGof7PaAK9d8HbYjdcFMgkWs6bzVkqlhc5CnZBr2XwtG5KveJJwr4CTXdM1Gwpm3xcPqQ17WzHl5wUGFbpMyiIaelqJzvEK2HJFYsCsD0Vy2sTJOOq2N3RLxPq-6Hg57XV8eYEvecB~uBhKcd7ga-ElUxTx7rT29uOvQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Molecular analysis for Phd2 CKO and Phd1/3 DKO mice. (A) Autoradiography of [35S]methionine-labeled PHD1, 2 and 3 produced by IVTT. Free, unincorporated [35S]methionine. (B) Western blotting analysis for PHD1, -2, and -3 in various tissues from wild-type adult mice. Equal amounts of IVTT-produced PHD1, -2, and -3 proteins were loaded as controls. Note PHD1 had 2 bands because of alternative translational initiation. (C) Anti-PHD2 Western blots for Phd1/3 DKO tissues. (D) Anti-HIF-1α and HIF-2α Western blots for Phd2 CKO and Phd1/3 DKO mice. (E,F) Q-PCR analysis for Epo mRNA. f/f, Phd2f/f; CKO, Phd2 CKO; WT, wild-type; DKO, Phd1/3 DKO. n = 6. (F) The scale in panel E was reduced. **P < .01; *P < .05. Error bars represent SEM.
