TβRII gene expression is subject to epigenetic silencing in PEL cells. (A) Methylation status at positions −25 and −140 of the TβRII promoter in PEL cell lines, BC1, BC3, and BCBL1, and Burkitt lymphoma cell line, Ramos, before and after 5-aza-dC treatment determined by methylation-specific PCR (U indicates unmethylated; M, methylated). (B) ChIP analysis for acetylated histone H4 and (C) Sp1 under the indicated conditions for 48 hours. (D) TβRII expression analyzed by real-time PCR and (E) Western blot after indicated treatments. Error bars represent SD.