LANA inhibits TβRII expression through epigenetic silencing. (A) Western blot for TβRII and phosphorylated Smad2/3 in KSHV+ LTCs and their uninfected TIVE cell counterparts in response to TGF-β1 treatment. (B) Expression of TβRII on induction of LANA in BJAB/Tet-on/LANA cells and (C) on siRNA knockdown of LANA in BC3 cells. (D) ChIP analysis of LANA association with the TβRII promoter. (E) Methylation status at positions −25 and −140 of the TβRII promoter in BJAB/Tet-on/LANA cells determined by methylation-specific PCR (U indicates unmethylated; M, methylated). (F) ChIP analysis of acetylated histone H4 and Sp1 at the TβRII promoter in BJAB/Tet-on/LANA cells.