Figure 3
Treatment with sorafenib affects FITC-dextran uptake by mature DCs. Monocytes were cultured with GM-CSF and IL-4 for 7 days. On day 5 of culture, sorafenib was added at the indicated concentrations (A,B). LPS (100 ng/mL) was added as a maturation stimulus for the last 24 hours (B). A total of 105 cells were incubated with FITC-dextran for 1 hour at 37°C and 4°C. The cells were washed and analyzed by FACS analysis. Open histograms represent FITC-dextran treated cells; solid histograms cells incubated in the absence of FITC-dextran. Mean fluorescence intensity of DCs labeled with FITC-dextran is shown.
