Expression of P2Y receptor mRNA in human PDCs. PDCs were isolated from PBMCs at high purity (> 95%) and mRNA was extracted immediately after isolation (ex vivo) or after 24-hour culture in the presence of IL-3 and CD40L to induce phenotypic PDC maturation. Profiles of P2Y receptor expression were generated by real-time RT-PCR. Results (mean ± SEM) of 3 independent experiments are given as relative units after normalization for differences to expression of the housekeeping gene HPRT. *P < .05.