EphrinB2 expression after ligation of the femoral artery. Femoral arteries from adult NMRI mice were analyzed proximal to the site of occlusion (A; scale bar represents 1 mm). Stenosis results in remodeling of the arterial vessel wall as evidenced by the increase in the number of nuclei in the media (B; n = 5, **P < .01 vs control). Staining of corresponding paraffin-embedded cross-sections revealed that endothelial cell ephrinB2 expression is very low under control and sham conditions but significantly up-regulated in the proximal part of the ligated artery (C; *P < .05 vs control, n = 5; scale bar represents 50 μm). The use of a control antibody did not yield any appreciable immunoreactivity (C, left image).