American Society of Hematology

Figure 1

$Figure 1. CLPs can be divided into Flk2+ and Flk2− fractions. (A) Strategy originally used to isolate CLPs by first gating on Lin−IL-7Rα+ cells (left panel) and subsequently on c-KitintSca-1int cells (middle panel) reveals heterogeneous expression of Flk2 (right panel). (B) Changing the gating strategy by first gating on lineage negative cells (not shown) reveals 2 distinct IL-7Rα+ populations (left panel). The Flk2+ fraction shows homogeneous expression of c-Kit and Sca-1 (middle panel) whereas the Flk2− fraction shows a more heterogeneous expression pattern (right panel). Numbers on plots are percentages of gated cells. (C) Surface expression of CD27, CD62L and CD93/AA4.1 on CLPF (bold lines) in comparison to MPP (solid thin lines), or isotype controls (dashed lines). (D) FACS plot overlay of CLPF (black dot plot) and lineage negative (excluding B220, gray contour plot) populations in C57Bl/6 BM. AA4.1hi cells are predominantly B220+.$

CLPs can be divided into Flk2⁺ and Flk2⁻ fractions. (A) Strategy originally used to isolate CLPs by first gating on Lin⁻IL-7Rα⁺ cells (left panel) and subsequently on c-Kit^intSca-1^int cells (middle panel) reveals heterogeneous expression of Flk2 (right panel). (B) Changing the gating strategy by first gating on lineage negative cells (not shown) reveals 2 distinct IL-7Rα⁺ populations (left panel). The Flk2⁺ fraction shows homogeneous expression of c-Kit and Sca-1 (middle panel) whereas the Flk2⁻ fraction shows a more heterogeneous expression pattern (right panel). Numbers on plots are percentages of gated cells. (C) Surface expression of CD27, CD62L and CD93/AA4.1 on CLP^F (bold lines) in comparison to MPP (solid thin lines), or isotype controls (dashed lines). (D) FACS plot overlay of CLP^F (black dot plot) and lineage negative (excluding B220, gray contour plot) populations in C57Bl/6 BM. AA4.1^hi cells are predominantly B220⁺.

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